Detection of pathogens in ixodid ticks collected from animals and vegetation in five regions of Ukraine

The distribution and prevalence of zoonotic pathogens infecting ixodid ticks in Western Europe have been extensively examined. However, data on tick-borne Eastern Europe, particularly Ukraine are scarce. objective the current study was, therefore, to investigate Anaplasma phagocytophilum, Anaplasmataceae, Rickettsia spp., Babesia Bartonella Borrelia burgdorferi sensu lato engorged questing collected from five administrative regions (oblasts) Ukraine, namely Chernivtsi, Khmelnytskyi, Kyiv, Ternopil, Vinnytsia. were both wild domestic animals vegetation. Of 524 collected, 3, 99, 422 identified as Ixodes hexagonus, ricinus, Dermacentor reticulatus, respectively. DNA samples individually extracted 168 354 adult subjected pathogen-specific PCR analyses. mean I. ricinus D. reticulatus were, respectively: 10 % (10/97) 3 (12/422) for A. phagocytophilum; 69 (67/97) 52 (220/422) members Anaplasmataceae family; 25 (24/97) 28 (117/422) spp.; (3/97) 1 (6/422) 9 (9/97) 5 (20/422) spp. Overall, between cities, there was no significant difference any respective (p > 0.05). B. s. l. varied 0 27 14–44%, respectively, with statistical significance cities In addition reporting updated Kyiv this is first provide prevalences This investigation also detect Neoehrlichia mikurensis Ukraine. These new will be useful medical veterinary practitioners well public health officials when diagnosing infections implementing measures combat diseases